Former Blades Harry Maguire, Kyle Walker and Aaron Ramsdale will all be present against the USA with England likely to field an unchanged line-up later today.
Both Walker and Ramsdale were unused substitutes in the victory against Iran, and will start in the same role against the USA unless there are any sudden injuries or selection issues.
Harry Maguire, who made 166 appearances for Sheffield United, will make his 50th appearance for England should he start as expected.
There had been doubts about the 29-year old after he was substituted in the victory against Iran as a precaution, after seemingly suffering the effects of a head clash.
Gareth Southgate explained however that the issue was actually dehydration in a pre-match press conference on Thursday, where he confirmed that both Maguire and captain Harry Kane are fit to play.
On Maguire, Southgate said: “It is great for Harry to get his 50th cap and it is well deserved.
“He is a major part of us getting two big major tournament performances and we want this to be the third.”
Sheffield United boast more of a presence in the current England squad than rivals Sheffield Wednesday – but more players have featured for England while playing for the Owls than the Blades.
Players featuring for Wednesday have earned 258 England caps while at the club, compared to 101 caps for United.
England will be hoping to beat the USA to progress into the Round of 16 of the World Cup, where they will play the second placed team in Group A if they manage to win their group.
The game will kick-off at 7pm and can be watched live on ITV1.
